25-OH Vitamin D Level Test Price and Details
0 labs availableLast Updated On Wednesday, June 18, 2025
What is 25-OH Vitamin D test?
The 25-OH Vitamin D test serves as a blood examination which measures your body's current vitamin D levels. Bonematuration and infectioncontrol and calciumregulation together with other essential functions depend on this vital vitamin. This evaluation shows whether an individual's vitamin D concentration is below normal or if the levels are deficient by revealing if levels are inside or outside the appropriate range.
The 25-Hydroxy vitamin D test provides essential information about your health status through measurements of body vitamin D levels to check for bone pain or muscle weakness or to protect general wellness.
This will help inform your health care practitioner to recommend nutritional or supplement changes for a level balance of vitamin D.
Why is 25 –Hydroxy Vitamin D Test Done?
Reasons for 25-Hydroxy Vitamin D Testing
The Vitamin D screening test is the best test that indicates how much vitamin D has been stored so far. The only time it is of any added use is when the signs or symptoms of a deficiency appear.
Your physician will prescribe vitamin D deficiency test if you have:
- Bone or joint pain
- Compromising infections
- Weakness and fatigue
- Muscle cramp or weakness
- Change of mood or depression
- Additionally, physicians may instruct you to undergo this test if you:
- Osteoporosis or bone mass that's too low
- Chronic liver or kidney disease
- Malabsorption syndromes (Crohn's disease or celiac disease, for example)
- Sun exposure risk factors that are too low
Who Should Get Tested?
Those with less sun exposure, darker skin color, older age, or on some medication (i.e., glucocorticoids or anticonvulsants) may want to be tested. Testing for 25-Hydroxy vitamin D is also preferable in those with personal or family history of vitamin D disease or autoimmune disease.
Test Procedure and Preparation
Is Fasting Necessary?
Fasting is not typically necessary before the 25-OH vitamin D interpretation test. It is a quick blood test that you can have done at any time of day with or without food. Since you are finding out how much 25-hydroxy vitamin D is in your blood and not how much sugar or cholesterol is in your blood, you hardly ever need to eat anything first.
Perhaps there would be a rule that went to certain doctors or to certain labs with regard to the amount that you do at once. That is, if you're performing a panel and you are also having some extra blood work done too (e.g., lipid panels or blood glucose), then perhaps you do have to fast. Always ask your physician or testing center for any preparation you must do before you are tested so that you will get good results and won't have to retake.
How the Blood Sample Is Taken?
Vitamin D is easy to have a blood test for:
- A health care worker will prepare your arm and put in a needle into a vein.
- A few drops of blood are taken.
- The sample is examined in a laboratory.
- The test normally does not exceed 5 minutes.
Normal and Abnormal 25-OH D Levels
Normal Vitamin D Range
Having your 25-OH D levels tested helps to know whether your you have normal vitamin D range, deficient, or insufficient.
Based on most guidelines:
- Deficient: Below 20 ng/mL (nanograms per milliliter)
- Insufficient: vitamin D insufficiency is 21–29 ng/mL
- Sufficient (Normal): 30–50 ng/mL
- Potentially Toxic: More than 100 ng/mL
What Low and High Levels Mean?
Low vitamin D will result in weakened bones, tiredness, weakened immunity, or heightened risk of fracture.
An excess of vitamin D is harmful and will result in nausea, kidney damage, or hypercalcemia (elevated levels of calcium in the blood).
Your doctor will take your 25-OH vitamin D test result together with your symptoms and signs, age, overall well-being, and other lab test results.
Symptoms and Causes of Vitamin D Deficiency
Early Warning Signs
Some of the common symptoms of deficiency of vitamin D are:
- Bone pain
- Cold or flu keeps recurring
- Mood swings or depression
- Loss of hair
- Wounds heal slowly
- Back pain
The symptoms build up stealthily and go unnoticed, hence the best is to get vitamin D checked occasionally.
Risk Factors
You are at greater risk of having a deficiency of vitamin D if:
- You are indoors most of the time
- You have darker skin color (melanin inhibits formation of vitamin D)
- You are obese or overweight
- You are over 65 years old
- You are a vegetarian
- You have digestive issues that lead to an inability to absorb nutrients
Vitamin D2 vs D3 Differences
Facts about Vitamin D3 vs D2 are still valid when using test results, as well as supplement choices:
- Vitamin D2 (ergocalciferol): Vegetarian and less effective at raising vitamin D levels.
- Vitamin D3 (cholecalciferol): Animal food and skin, product of sun exposure. More effective and lasts longer in the body.
Most physicians prescribe vitamin D3 supplements with improved absorption and efficacy.
25-OH Vitamin D Test Price in Pakistan
The prices of the tests for vitamin D in Pakistan are also not uniform for different cities and labs. It is around PKR 2,000 to PKR 4,000. Package prices for overall vitamin profiles and some centers for home sampling are also offered in some laboratories.
Prices also vary a little from city to city, i.e., Lahore, Karachi, Islamabad, etc., therefore it is always advisable to call at the local labs or order online.
How to Book a Vitamin D Test with InstaCare?
Booking your 25-hydroxy vitamin D test with InstaCare is easy. Simply follow these steps if you want to know how to increase vitamin D:
- Visit the InstaCare website or mobile app.
- Select the 25-OH vitamin D test.
- Select your city and diagnostic lab.
- Home. test or go to the lab.
- Select your choice.
- Book online and pay for an appointment.
You can receive your vitamin D blood test result online, either 24 to 48 hours depending on the lab.
Conclusion
25-OH vitamin D blood test is a simple way to know what your body needs from vitamin D. If you experience chronic fatigue, bone aches, or simply want to be healthy and disease-free, the test will come in handy. Knowing your 25-OH D levels, catching signs of deficiency early, and how to enhance them could be a lifesaver for your health.
With affordable testing in Pakistan and easy InstaCare booking, no reason to put off your vitamin D test. Take action now, get tested, educate yourself, and take control of your health.
FAQs
What is the 25-OH Vitamin D test?
The test is calibrated for the amount of total 25-hydroxy vitamin D (D2 and D3) in your blood to measure the level of your vitamin D in the body and identify deficiency or toxicity.
How does normal vitamin D appear?
Normal 25-OH D level normally is 30–50 ng/mL. Less than 20 ng/mL is subdeficiency, more than 100 ng/mL is toxic.
Well, of course, how does one raise his/her vitamin D level?
Vitamin D is raised by 10–30 minutes sunbathing daily, eating a vitamin D-containing food (fat fish, eggs, vitamin D milk), or vitamin D3 supplements after consulting your physician.
Frequently Asked Questions
The 25-OH Vitamin D Level test is a medical diagnosis of your health which is conducted by healthcare laboratories. In this test, the lab will collect your 25-OH Vitamin D Level sample and will process it in its facility. Reporting time of 25-OH Vitamin D Level test is 2 Day(s). After the processing results will be published and you will be notified. You can either download your lab test report online or can visit the near sample collection center to get printed reports.
Medical tests are the procedures in which a laboratory takes a sample of your blood, urine, or any other body fluid or body tissue to get detailed information about your health. Usually, lab tests are used to help to diagnose and screening specific diseases or medical conditions. Some tests are also used to gain general information about organs and body systems.
The price of 25-OH Vitamin D Level tests varies from lab to lab but on InstaCare you will find discounts on the tests. Price range of 25-OH Vitamin D Level test is 0 - 0.
Yes, You can view the results of your tests online with InstaCare and its partners.
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